Plant-expression System for Recombinant Vaccine Production : Future Design Prevention of Poultry Viral Diseases in Indonesia.

Mar28

Accepted Paper Indonesian Student Scientific Conference PPI Wageningen, Belanda

ABSTRACT

Different types of chicken diseases have been reported occurrence in Indonesia, such as Avian Influenza (AI), Newcastle Disease (ND), Infectious Bursal Disease (IBD), and Invectious Bronchitis (IB) in sectors 3 and 4 sector 3 is a breeder who has poultry population between 1000-20000 poultry and Sector 4 with a population of 1-100 poultry1. One example, the percentage of cases of AI almost every province in Indonesia, ranging from West Java, Central Java, East Java, Sumatra and Sulawesi the highest incidence2. Avian influenza (AI), Newcastle Disease (ND), Infectious Bursal Disease (IBD), and Invectious Bronchitis (IB) should also remain aware of the poultry, especially chickens . Avian influenza (AI) belonging to the type of pests and diseases of quarantine animals while group 1, Newcastle Disease (ND), Infectious Bursal Disease (IBD), and Invectious Bronchitis (IB) included in group 23. Vaccines are one of the important weapon in controlling the disease. Therefore, assessment of vaccine should be continuously carried out to ascertain whether the vaccine still provides excellent protection4.

Various types of vaccines have been developed to address and prevent the transmission of infectious diseases. Several approaches vaccine design technology has been progressing rapidly enough to overcome the problems associated with the complexity of infectious disease control. One of these uses is done at the level of DNA vaccine5. Edible vaccines is one of the applications of vaccine derived from the DNA. Bacterial or viral DNA fragments were cloned into a plant a gene that would encode a protein formation, which is usually selected proteins located on the surface of bacterial cells or viruses, so that when the plant is consumed will produce an immune response. Formed immune system will be able to recognize specific epitopes on the cell surface of bacteria and viruses, which enter the body, so it will be protected from infection by bacteria or viruses6.

Molecular farming of pharmaceuticals in plants has the potential to provide almost unlimited amounts of recombinant proteins for use in disease diagnosis, prevention or treatment7. Oral delivery of plant-made vaccines is particularly subject attractive due to Reviews their exceptional advantages. This led to an incredible investment in the field of transgenic plant vaccines for veterinary purpose8. Plants for vaccine testing has been done on several avian diseases such as avian influenza, newcastle disease, infectious bursal disease and infectious bronchitis9.10.11.12.

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Figure 1. Strategies for expression of antigens in plants

In addition, passive immunization with oral administration of antibodies expressed in transgenic plants against avian coccidiosis is an innovative strategy. Plants have been identi fi ed as promising expression systems for commercial production of vaccine antigens. In phase I clinical trials Several plant-derived vaccine antigens have been found to be safe and induce sufficiently high immune response. This Spake, transgenic plants, Including edible plant parts are suggested as excellent alternatives for the production of vaccines and economic scale-up through cultivation13. This experiment may pave the way for the development of edible animals promising vaccine in the near future14. it seems plausible for plant-based vaccine is to have a niche in veterinary field. Plants can be an adequate system of choice to produce immunogens against and suggest the utility of our plant-expression system for recombinant vaccine production15.16.

 

 

Keywords: Plant-expression, Recombinant Vaccine, Poultry Viral Diseases, Indonesia.

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  1. Adjid, R.M.A, et al. 2006. Dukungan teknologi veteriner dan strategi pengendalian penyakit unggas (ayam) di sektor 3 dan 4. Puslitbang Peternakan : 22-27
  2. Anonymous. 2012. Dinamika Penyakit Ayam di 2012 dan Prediksinya di 2013. Artikel info.medion.co.id
  3. KEMENTAN. 2009. karantina.deptan.go.id
  4. Anonimous. 2010. Virus AI Terus Berubah dan Masih Mengancam Kita. Artikel info.medion.co.id
  5. Radji, Maksum. 2009. Vaksin Dna: Vaksin Generasi Keempat. Majalah Ilmu Kefarmasian, Vol. VI, No. 1 : 28 – 37.
  6.  Radji, Maksum. 2004. Pemberian Vaksin Melalui Tanaman Transgenik. Majalah Ilmu Kefarmasian, Vol. I, No.1:1-9.

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